• PGK (PGK promoter): 1438–1937 ... Figure 4. pMSCV Seq/PCR Primer sequences Location of Sequencing Primers (for all MSCV Vectors) pMSCVneo Vector
  • • CMV promoter : nt234- 802 • Multiple cloning sites: nt880 -946 • PGK promoter transcription cassette: nt1159-3409 • WPRE sequence: 3420- 4008 Note: 1. Since lentiviral transfer vector may undergo sequence re -arrangement and/or deletion when its ligated products were transformed into DH5 α competent
  • Promoter: Cytomegalovirus (CMV) immediate early promoter and the mouse phosphoglycerate kinase promoter (PGK) Purpose: This plasmid contains two promoters that terminate transcription at the same poly-adenylation signal, allowing the expression of two genes from one expression cassette, where the second gene is the puromycin resistance gene ...
  • Pgk-1 is an X-linked gene encoding 3-phosphoglycerate kinase, an enzyme necessary in every cell for glycolysis. The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, a potential binding site for the Sp1 transcription factor, a CCAAT sequence, but no TATA box.
  • Negative inducible promoter pBad is another popular prokaryotic promoter often used for bacterial protein purification. When arabinose is absent, regulatory protein AraC binds O and I1 sites upstream of pBad, blocking transcription. The addition of arabinose causes AraC to bind I1 and I2 sites, allowing transcription to begin.
  • Sequence Details Sequence The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C.Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
PGK promoter in the E. coli strain makes this promoter a suitable tool for our purpose. In this study, we built up a novel Cre recombinase-mediated in vivo mcDNA (CRIM) platform for the production of mcDNA in vivo based on the presence of Cre recombi-nase driven by the PGK promoter. The plasmid could replicate as a complete parental
The vectors also contain a promoter that is active in ES cells [such as that of the mouse phosphoglyceratekinase (Pgk) gene] followed by a first exon (such as that of the Bruton’s Tyrosine Kinase (Btk) gene) upstream of a splice donor (SD) signal.
sequence yeast promoter dna coding sequence Prior art date 1989-10-18 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Expired - Lifetime Application number JP28055790A Other languages Japanese (ja) Other ... The following sequence of eGFP was selected to use in the oligonucleotide: 5'-GCTGACCCTGAAGTTCATCT-3' The cells were selected with medium containing 2 'xg/m' puro and were assayed after 8 d. siRNA-mediated inhibition of gene expression via pgk-1 promoter in PI 9 EC cells To determine whether siRNA could affect the pgk-1 promoter, we next used ...
Misc.Comments : The promoter and terminator sequences were derived from the phosphoglycerate kinase gene. Saccharomyces cerevisiae containing a single copy of the neomycin phosphotransferase gene are sensitive to G418; resistance to 400 ug/ml is expressed if there are several copies per cell.
Effectively, CMV is a very bad promoter for a long term expression in stem cells, PGK or EF1 work better. If you want to test different promoters without annoying you to make a lot of clonages, I ... The dual promoter phiC31 vectors are available in two formats: The pFC-EF1-MCS- PGK-RFP-Puro vector (cat# FC550A-1-SBI) has EF1a driving your gene´s expression cloned into the MCS and PGK expressing the RFP-Puro combo markers.
downstream sequence translation french, English - French dictionary, meaning, see also 'downmarket',downs',down-market',downstairs', example of use, definition ... Aug 16, 2012 · To this end, two vectors were used, a standard vector with the T7 phage promoter and an introduced sequence encoding PA-4D (pGPA), and a modified vector containing the sequence encoding PA-4D as a part of the expression cassette (pGKPA). We used two different selection markers: CmR as the Type I marker and KmR as the Type II marker.

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