sequence yeast promoter dna coding sequence Prior art date 1989-10-18 Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Expired - Lifetime Application number JP28055790A Other languages Japanese (ja) Other ... The following sequence of eGFP was selected to use in the oligonucleotide: 5'-GCTGACCCTGAAGTTCATCT-3' The cells were selected with medium containing 2 'xg/m' puro and were assayed after 8 d. siRNA-mediated inhibition of gene expression via pgk-1 promoter in PI 9 EC cells To determine whether siRNA could affect the pgk-1 promoter, we next used ...
Effectively, CMV is a very bad promoter for a long term expression in stem cells, PGK or EF1 work better. If you want to test different promoters without annoying you to make a lot of clonages, I ... The dual promoter phiC31 vectors are available in two formats: The pFC-EF1-MCS- PGK-RFP-Puro vector (cat# FC550A-1-SBI) has EF1a driving your gene´s expression cloned into the MCS and PGK expressing the RFP-Puro combo markers.
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