Next, the cellular ROS level was measured by flow cytometry as described previously . As shown in Figure 1(c) , the populations in higher fluorescence intensities significantly increased by ~10% after the exposure of DpdtpA to the cells for 24 h, but the addition of NAC, a ROS scavenger, significantly decreased ROS production (~23%), hinting ... Jul 27, 2012 · A reduction in the interaction of the two clones with the macrophages was detected by flow cytometry. Monoclonal antibodies were produced and used to demonstrate the presence of these proteins on the bacterial surface of the corresponding clone, and on the H. parasuis phagocytosis-resistant strain PC4-6P.
My knowledge on flow cytometry is limited. So that it would be great if you are able to recommend some paper or protocols to follow especially these kind of living staining. Thanks in advance. The fluorescence of LysoTracker reflects the amount of lysosomes. Flow cytometry plots with median fluorescence and diagrams with fluorescence intensity related to DMSO-treated cells (0 µM Ecdy) are depicted. Results are calculated as the mean ± SEM of three experiments. *p < 0.05; **p < 0.01.
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