counted from 4 peripheral and 4 central fields (0.18 mm2)peranimal (CTL: n54; CKO: n55) using BioQuant Nova Software (BIOQUANT Image Analysis Corporation, Nashville, TN). Confocal micrographs of 4 peripheral and 4 central fields (0.10 mm2) were taken from 3 CTL reti-nas, to identify all Cre-positive, Sox9-positive, and double-labeled cell much better way is to use nullable in Laravel 5.5 validations . Ref Docs. The field under validation may be null. This is particularly useful when validating primitive such as strings and integers that can contain null values.
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