The proteins have hydrophilic amino acids in contact with the water on the outside of membranes, and hydrophobic amino acids in contact with the fatty chains inside the membrane. Proteins comprise about 50% of the mass of membranes, and are responsible for most of the membrane's properties. The critical contribution of membrane proteins in normal cellular function makes their detailed structure and functional analysis essential. Detergents, amphipathic agents with the ability to maintain membrane proteins in a soluble state in aqueous solution, have key roles in membrane protein manipulation.
The integral proteins are amphipathic molecules, i.e. within the same molecule hydrophilic and hydrophobic groups occur. The hydrophilic groups protrude from the surface while the hydrophobic groups remain embedded in the lipids (Fig. 1.12). Schematic Representation of Molecular Organization of the Plasma Membrane. Functions of Plasma Membrane: ¾Requires the use of carrier proteins (transport proteins that physically bind to the substance being transported). Objective 17a We will examine 2 types of active transport: transport: membrane pumpsmembrane pumps (protein(protein--mediated active transport) and mediated active transport) and coupled coupled transporttransport (cotransport).
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